Ko te Proteinase K CAS 39450-01-6, he mea hoko pai rawa atu i te ao, he mea rongonui i te wheketere, he tere te pānga, ā, ko te rā whakaputa hou rawa atu.
E whakanuia ana e ia mema o tā mātou kapa mahi nui te nui o ngā moni whiwhi, ngā whakaritenga a ngā kiritaki me ngā whakawhitiwhiti kōrero a te whakahaere mō te hoko pai rawa atu i te wheketere. He rongonui puta noa i te ao.Pūmua K CAS 39450-01-6He nui te tere o te pānga me te rā whakaputa hou, i tēnei wā, e tumanako ana mātou ki te mahi tahi nui atu me ngā kiritaki o tāwāhi i runga i ngā painga tahi. Kia mahara kei te kore utu koe ki te whakapā mai ki a mātou mō ētahi atu kōrero.
E whakanuia ana e ia mema o tā mātou kapa nui o ngā moni whiwhi mahi ngā whakaritenga a ngā kiritaki me ngā whakawhitiwhiti kōrero a te whakahaere mōHaina 39450-01-6, E mihi ana mātou ki tō tautoko, ā, ka mahi mātou ki ā mātou kiritaki i te kāinga me tāwāhi me ngā taonga kounga teitei me te ratonga tino pai e hāngai ana ki te whanaketanga tonu. E whakapono ana mātou ka whai hua koe i tō mātou ngaiotanga a mua tata nei.
| Wai Nama 1 | |
| Ko te ngohe motuhake ki te pūmua >800U/mL, ko te kukū he 20mg/mL, kāore he toenga Nickase | |
| Paura Nama 2 | |
| Āhua | Paura mā kua whakapūkaratia |
| Te parakore hiko | ≥95% |
| Te mahi a te whākōkī | ≥30U/mg |
| Ngā toenga waikawa karihi | Kāore e kitea |
| Te toenga o te deoxyribonuclease | Kāore e kitea |
| Ngā toenga ribonuclease | Kāore e kitea |
| Tau Kete | G1205-10ML |
| Rokiroki | Ina rongoatia ki te –20 °C, ka mau tonu te mahi a te hua mō te iti rawa 1 tau, ka taea te kawe i roto i te putea hukapapa. |
Pūmua KHe protease serine tēnei nō te whānau subtilisin, he pai te mahi whākōkī me te whānui o te motuhaketanga o te papa, e wehewehe ana i ngā here ester me ngā peptide e tata ana ki te pito-C o ngā waikawa amino hydrophobic, ngā waikawa amino kei roto he whanariki, me ngā here waikawa amino kakara, e whakamahia ana hei wawahi i ngā pūmua hei whakaputa peptide poto.Pūmua KKei a ia te āhuatanga o te toru whakakorikori noa o Asp39-His69-Ser224, he mea ahurei ki ngā serine proteases, ā, e rua ngā pae here Ca2+ huri noa i te pokapū hohe hei whakanui ake i tōna pumau me te pupuri i te kaha whākōkī teitei i raro i te whānuitanga o ngā āhuatanga.
1. Kete tātaritanga ira.
2. Ngā kete tango RNA me te DNA.
3. Tangohia ngā wāhanga kore-pūmua i roto i ngā kiko, ā, whakangaromia ngā poke pūmua (pērā i te whakarite i ngā kano kano DNA me te heparin).
4. Te Whakarite i te DNA Chromosomal mō te Pulse Electrophoresis.
5. Te whakakore i te uru.
6. Te whakawhanaketanga me te whakaputa i ngā matū whakapūmua albumin glycated whākōkī i roto i te mara o te tātaritanga in vitro.
1. 30mg/pōta 1g/pōta, 100g rānei/pōta
2. 1kgs/pēke pepa konutea
He pūmua serine pumau te Proteinase K, he whānui te motuhaketanga o te matūriki. Ka whakaitihia e ia te maha o ngā pūmua i roto i te āhua taketake ahakoa kei te aroaro o ngā hopi horoi. Kāore e whakahohehia e ngā katote konganuku, ngā kaihoko chelating (hei tauira, EDTA), ngā matū sulfhydryl, e ngā aukati tripsin, chymotrypsin rānei. He pumau i runga i te whānuitanga pH whānui (4–12.5), me te mahi tino pai i te pH 6.5–9.5. Ka taea te whakaoho i te mahi mā te tāpiri i ngā kaihoko denaturing (SDS me te Urea). Ka puta te denaturation tere o te whākōkī i te pāmahana neke atu i te 70°C. Ka piki haere te autolysis o te whākōkī i te pH kawakore. Heoi, kāore te Proteinase K e whakakorea katoatia e te autolysis. Kei te mau tonu te mahi proteolytic katoa o ētahi kongakonga whākōkī, ahakoa i muri i te autolysis nui. He maha te whakamahinga o te Proteinase K i roto i ngā tono koiora ngota hei keri i ngā pūmua kāore e hiahiatia, pērā i ngā nucleases i roto i ngā whakaritenga DNA, RNA rānei mai i ngā moroiti, ngā pūtau whakatipu, me ngā tipu. Ko te whākōkī e whakamahia ana i te 50–200 ug/ml i roto i ngā whakaritenga waikawa karihi i te pH 7.5–8.0 me te 37~55oC. Ka rerekē ngā wā whakatipu mai i te 30 meneti ki te 18 hāora.
He pūmua serine pumau, he tino tauhohe hoki; e whakamahia ana mō te wehewehe i te pūmua me te waikawa karihi. Ka whakamahia te Pūmua K hei pure i te RNA me te DNA mai i ngā kiko, i ngā raina pūtau rānei.
E whakanuia ana e ia mema o tā mātou kapa mahi nui te nui o ngā moni whiwhi, ngā whakaritenga a ngā kiritaki me ngā whakawhitiwhiti kōrero a te whakahaere mō te Proteinase K CAS rongonui rawa atu o te wheketere.39450-01-6He nui te tere o te pānga me te rā whakaputa hou, i tēnei wā, e tumanako ana mātou ki te mahi tahi nui atu me ngā kiritaki o tāwāhi i runga i ngā painga tahi. Kia mahara kei te kore utu koe ki te whakapā mai ki a mātou mō ētahi atu kōrero.
Te hoko pai rawa atu i te wheketereHaina 39450-01-6Ka mihi nui mātou ki tō tautoko mai, ā, ka mahi mātou ki ā mātou kiritaki i te kāinga me tāwāhi me ngā taonga kounga teitei me te ratonga tino pai e hangai ana ki te whanaketanga tonu. E whakapono ana mātou ka whai hua koe i tō mātou ngaiotanga a mua tata nei.
