Pūmua Ma Pūmua Ma Teitei, Proteinase K CAS 39450-01-6, Tukunga Wheketere i roto i te Hokonga Wera i te Utu Iti Rawa
E piri tonu ana ki tō whakaaro "Te waihanga i ngā otinga kounga teitei me te hanga hoa ki ngā tāngata o te ao katoa", ka whakatakoto tonu mātou i te hiahia o ngā kiritaki ki te tīmata me te Paura Ma Parakore Teitei e Tukuna ana e te wheketere.Pūmua K CAS 39450-01-6I roto i te Hokonga Wera i te Utu Iti Rawa, He mahi tahi hohonu tā mātou me ngā rau o ngā wheketere e tata ana ki Haina. Ka taea e ngā taonga e tukuna ana e mātou te ōrite ki ngā momo tono rerekē. Kōwhiria mātou, ā, e kore mātou e mea i a koe kia pouri!
E piri tonu ana ki tō whakaaro mō te "Waihanga i ngā otinga kounga teitei me te hanga hoa ki ngā tāngata o te ao katoa", ka whakatakoto tonu mātou i te hiahia o ngā kiritaki ki te tīmata me tePaura Proteinase K /Fluvoxamine me te Proteinase K o Haina, Ko tā mātou kaupapa matua ko te "pono tuatahi, kounga pai rawa atu". Inaianei kei a mātou te māia ki te whakarato ratonga pai rawa atu me ngā taonga tino pai ki a koe. Ko tā mātou e tino tumanako nei ka taea e mātou te whakatū i te mahi tahi pakihi toa-toa ki a koe a muri ake nei!
| Wai Nama 1 | |
| Ko te ngohe motuhake ki te pūmua >800U/mL, ko te kukū he 20mg/mL, kāore he toenga Nickase | |
| Paura Nama 2 | |
| Āhua | Paura mā kua whakapūkaratia |
| Te parakore hiko | ≥95% |
| Te mahi a te whākōkī | ≥30U/mg |
| Ngā toenga waikawa karihi | Kāore e kitea |
| Te toenga o te deoxyribonuclease | Kāore e kitea |
| Ngā toenga ribonuclease | Kāore e kitea |
| Tau Kete | G1205-10ML |
| Rokiroki | Ina rongoatia ki te –20 °C, ka mau tonu te mahi a te hua mō te iti rawa 1 tau, ka taea te kawe i roto i te putea hukapapa. |
Pūmua KHe protease serine nō te whānau subtilisin te Proteinase K, he pai te mahi whākōkī me te whānui o te motuhaketanga o te substrate, e wehewehe ana i ngā here ester me ngā peptide e tata ana ki te C-terminus o ngā waikawa amino hydrophobic, ngā waikawa amino kei roto te whanariki, me ngā here waikawa amino kakara, e whakamahia ana hei wawahi i ngā pūmua hei whakaputa peptide poto. Kei a Proteinase K te āhuatanga o te triad catalytic noa Asp39-His69-Ser224 ahurei ki ngā protease serine, ā, e rua ngā pae here Ca2+ huri noa i te pokapū hohe hei whakanui ake i tōna pumau me te pupuri i te mahi whākōkī teitei i raro i te whānuitanga o ngā āhuatanga.
1. Kete tātaritanga ira.
2. Ngā kete tango RNA me te DNA.
3. Tangohia ngā wāhanga kore-pūmua i roto i ngā kiko, ā, whakangaromia ngā poke pūmua (pērā i te whakarite i ngā kano kano DNA me te heparin).
4. Te Whakarite i te DNA Chromosomal mō te Pulse Electrophoresis.
5. Te whakakore i te uru.
6. Te whakawhanaketanga me te whakaputa i ngā matū whakapūmua albumin glycated whākōkī i roto i te mara o te tātaritanga in vitro.
1. 30mg/pōta 1g/pōta, 100g rānei/pōta
2. 1kgs/pēke pepa konutea
He pūmua serine pumau te Proteinase K, he whānui te motuhaketanga o te matūriki. Ka whakaitihia e ia te maha o ngā pūmua i roto i te āhua taketake ahakoa kei te aroaro o ngā hopi horoi. Kāore e whakahohehia e ngā katote konganuku, ngā kaihoko chelating (hei tauira, EDTA), ngā matū sulfhydryl, e ngā aukati tripsin, chymotrypsin rānei. He pumau i runga i te whānuitanga pH whānui (4–12.5), me te mahi tino pai i te pH 6.5–9.5. Ka taea te whakaoho i te mahi mā te tāpiri i ngā kaihoko denaturing (SDS me te Urea). Ka puta te denaturation tere o te whākōkī i te pāmahana neke atu i te 70°C. Ka piki haere te autolysis o te whākōkī i te pH kawakore. Heoi, kāore te Proteinase K e whakakorea katoatia e te autolysis. Kei te mau tonu te mahi proteolytic katoa o ētahi kongakonga whākōkī, ahakoa i muri i te autolysis nui. He maha te whakamahinga o te Proteinase K i roto i ngā tono koiora ngota hei keri i ngā pūmua kāore e hiahiatia, pērā i ngā nucleases i roto i ngā whakaritenga DNA, RNA rānei mai i ngā moroiti, ngā pūtau whakatipu, me ngā tipu. Ko te whākōkī e whakamahia ana i te 50–200 ug/ml i roto i ngā whakaritenga waikawa karihi i te pH 7.5–8.0 me te 37~55oC. Ka rerekē ngā wā whakatipu mai i te 30 meneti ki te 18 hāora.
He pūmua serine pumau, he tino tauhohe hoki; e whakamahia ana mō te wehewehe i te pūmua me te waikawa karihi. Ka whakamahia te Pūmua K hei pure i te RNA me te DNA mai i ngā kiko, i ngā raina pūtau rānei.
E piri tonu ana ki tō whakaaro "Te waihanga i ngā otinga kounga teitei me te hanga hoa ki ngā tāngata o te ao katoa", ka whakatakoto tonu mātou i te hiahia o ngā kiritaki ki te tīmata me te Factory Supply High Purity White Powder Proteinase K CAS 39450-01-6 i roto i te Hokonga Wera i te Utu Iti rawa, He mahi tahi hohonu tā mātou me ngā rau o ngā wheketere e tata ana ki Haina. Ka taea e ngā taonga e tukuna ana e mātou te ōrite ki ngā tono rerekē. Kōwhiria mātou, ā, e kore mātou e mea i a koe kia pouri!
Tukunga WheketerePaura Proteinase K /Fluvoxamine me te Proteinase K o Haina, Ko tā mātou kaupapa matua ko te "pono tuatahi, kounga pai rawa atu". Inaianei kei a mātou te māia ki te whakarato ratonga pai rawa atu me ngā taonga tino pai ki a koe. Ko tā mātou e tino tumanako nei ka taea e mātou te whakatū i te mahi tahi pakihi toa-toa ki a koe a muri ake nei!
